Coding
Part:BBa_K2637008:Design
Designed by: Haoqian Zhao Group: iGEM18_Tianjin (2018-10-05)
BD-DNA-binding Domain of Gal4 transcription factors
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 234
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 153
Design Notes
We performed codon optimization on this gene sequence to use it in yeast. And then we modified some sites to deleted some enzyme cutting sites.
Source
BD is from yeast genome.