Coding

Part:BBa_K2637008:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)


BD-DNA-binding Domain of Gal4 transcription factors


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 234
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 153


Design Notes

We performed codon optimization on this gene sequence to use it in yeast. And then we modified some sites to deleted some enzyme cutting sites.

Source

BD is from yeast genome.

References